Scientific Paper & FrontPage Assignment-4

You need to give sufficient details of your work so that   someone else can repeat what you have done. Independent repeatability is one of the Hallmarks of science.
                  Remember "Cold Fusion"?

Specimens of Webbium grandium (Webb) were collected from the campus of the University of Subway at Subiatropilis. These were placed into a fixative, antifreeze solution that contained 10% Dimethylsulfoxide, 4% Formaldehyde (w/v), 1% Tween 20 and 0.05 M Sodium Cacodylate. The pH was set at 7.5. Samples were stored at 5 degrees C for approximately 3 weeks. Specimens were mounted on stubs in a freezing microtome and sectioned at 50 - 30 microns. These were stained with Toluidine Blue (0.05%) or Phloroglucinol (saturated solution in 20% HCl). The freezing support medium (Tissue Freezing Medium, Triangle Biomedical Sciences) was diluted with water and removed by blotting prior to staining because it formed a precipitate with both stains. Unstained specimens were mounted in the freezing support medium and viewed without further treatments.

Specimens were viewed and photographed with bright-field and polarized optics. One polarizer was always in the light path above the specimen and the other was placed over the field diaphragm and rotated to achieve full or partial polarization. Photographs were made with Kodak Ektachrome slide film with an ASA rating of 100. Slides were digitized with a Hewlett Packard PhotoSmart scanner at 1200 dots/inch. These were processed with Adobe Photo Deluxe. Sigma Scan Image (Jandel Sci.) was used to take measurements and generate basic statistics. Web pages were created with FrontPage 98.