Three-dimensional Reconstruction of the Drosophila Larval and Adult Brain

Karlheinz Rein *1, Peter Robin Hiesinger *2, Malte Zöckler 3, Jan Kirsten 1, Karl-Friedrich Fischbach 2 and Martin Heisenberg 1

* The first two authors contributed equally to this work
1 University of Würzburg, Lehrstuhl für Genetik, Biozentrum, Am Hubland, 97074 Wuerzburg, rein@biozentrum.uni-wuerzburg.de
2 University of Freiburg, Institute for Biology III, Schaenzlestr. 1, D-79104 Freiburg, Germany, robin@biologie.uni-freiburg.de
3 Konrad-Zuse-Zentrum für Informationstechnik, Takustr. 7, D-14195 Berlin-Dahlem, zoeckler@zib.de

Overview

Here we present reconstructions of individual wild type Drosophila larval and adult brains. To obtain these, whole brains were dissected from heads and labeled fluorescently. Using a confocal microscope we obtained about 200 optical sections (thickness a bout 1 µm) for each brain. The individual brain structures in these sections were labeled manually and surfaces were generated. We brought the surfaces and parts of the original image data into the VRML format, so that they can be viewed on normal desktop computers using a web-browser and a freely available plug-in. Movies showing the brains from different orientations were generated using volume visualization techniques.



Inhaltsverzeichnis der CD