Three-dimensional Reconstruction of the Drosophila Larval and Adult Brain
Karlheinz Rein *1, Peter Robin Hiesinger *2, Malte Zöckler
3, Jan Kirsten
1, Karl-Friedrich Fischbach 2 and Martin Heisenberg 1
* The first two authors contributed equally to this work
1 University of Würzburg, Lehrstuhl für Genetik, Biozentrum, Am
Hubland, 97074 Wuerzburg, rein@biozentrum.uni-wuerzburg.de
2 University of Freiburg, Institute for Biology III, Schaenzlestr. 1, D-79104
Freiburg, Germany, robin@biologie.uni-freiburg.de
3 Konrad-Zuse-Zentrum für Informationstechnik, Takustr. 7, D-14195
Berlin-Dahlem, zoeckler@zib.de
Overview
Here we present reconstructions of individual
wild type Drosophila larval and adult brains. To obtain these, whole brains were
dissected
from heads and labeled fluorescently. Using a confocal microscope we obtained about 200
optical sections (thickness a bout 1 µm) for each brain. The individual brain structures in
these sections were labeled manually and surfaces were generated. We brought the surfaces and
parts of the original image data into the VRML format, so that they can be viewed on normal
desktop computers using a web-browser and a freely available plug-in. Movies showing the
brains from different orientations were generated using volume visualization techniques.
Inhaltsverzeichnis der CD