Primary-Secondary Growth Stems-6

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Coleus-2240Lab.jpg (35656 bytes)
Corner of Coleus Stem stained with Phloroglucinol. The Phloem has not stained but the Xylem, and Phloem Fibers have stained positively for lignin. The dark layer in the Cortex is Chlorenchyma.
Coleus4240Lab.jpg (38621 bytes)
Same as above but viewed with crossed Polarizers. Note the Collenchyma which was invisible with bright-field optics.

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