|
|
|
|
|
| C O N T E N T S |
| C-Fern Home |
| Web Manual |
| Introduction C-Fern Nutrient Medium Culture Conditions Gametophyte Culture Gametophyte Observations Sporophyte Culture Spore Mutagenesis Bibliography |
|
Light Stand Growth Pod DNA Extraction C-Fern T-shirt Who's Who Selection and mutants Media formulation error Image gallery |
| Web Journal |
| Educational Resources |
|
National Science Standards Gametophyte development Student research questions Research in the classroom Research with C-Fern Brief description of C-Fern C-Fern manipulation Root tropism? |
| Frequently Asked Questions |
| Workshop and Exhibit Schedule |
| Educational
Materials |
| Investigations Mutant & wild type spores Manual & Culture Instructions Supplies - Domes, media |
|
 |
| Development
of C-Fern is supported by the National Science Foundation (NSF-DUE) |
|
|
|
Copyright
© 1997-2000
Thomas R. Warne and Leslie G. Hickok. All rights reserved. |
|
|
C-Fern
Web Manual - Spore Mutagenesis
Materials:
Procedures:
1. Soak spores for 24 h prior to sterilization and irradiation.
2. Sterilize using standard methods and place sterile spore suspension in
a glass petri dish. Place dish inside plastic sandwich bag.
3. Irradiate spores with 25,000 Roentgens or about 555 Roentgens min-1 for
45 min. After irradiation sow spores as usual.
Under these conditions, spore germination at 10 days following sowing is
about 80%; a screen with 10-5 M flurodeoxyuridine gives a mutant frequency
of 2 X 10-5.
|
