Annotated Horizontal Autofluorescent Sections
Sixteen 7 µm horizontal paraffin sections of male flies. Autofluorescence was imaged using a confocal microscope at 1024 x 1024 resolution. Images are provided in JPEG format here, a series of high resolution GIF versions is also available. Horizontal sections are arranged from dorsal to ventral.
Characterization of Gal4 lines with expression in the Drosophila optic lobe usin
We have used the Gal4/UAS targeted expression system to analyze the regional specificity and developmental dynamics of reporter gene expression in a collection of enhancer trap lines. These lines express the yeast transcription factor Gal4 in subsets of cells within the optic lobe of Drosophila. As a reporter, we used a tau-GFP fusion construct (1). This fusion protein is transported into axonal processes due to the microtubuli-binding properties of the tau protein. It offers a high level of resolution in structural analysis. Due to its natural fluorescence, it is ideally suited for combination with antibody stains for two-channel confocal laser scan microscopy.
Columnar lamina-medulla connections in structural brain mutants
This poster describes some important facets of the small optic lobes and irreC phenotypes by use of the Golgi method. Emphasis is on the columnar neurons that connect retina and lamina with the medulla.