ibclogo XVI International Botanical Congess

Abstract Number: 6091
Session = 4.14.3


CONTROL OF CHANGES IN CELL CYCLE ACTIVITIES IN SHOOT MERISTEMS


A. Jacqmard1 & D. Inze2, 1Plant Phys, Dept. Botany, B22 Univ. Liege, B4000 Liege, 2Lab Genetika, Dept. Genetics, Univ. Ghent, B9000 Ghent, Belgium


The shoot apical bud of 2-month-old vegetative plants of Arabidopsis thaliana is used to gain insight into the specific roles of cell cycle genes in the mitotic and endoreduplication cycles. Expression of cell cycle genes is analyzed by in situ hybridization. This bud is composed of various kinds of tissues differing in their states of proliferation and differentiation. Mitotic divisions are observed in the meristem, young leaf primordia and cambial cells of maturing leaves. Coinciding with the specification of mesophyl cell identity in leaf primordia and specification of pith cell identity in stem, mitosis switches to endore duplication.Our data indicate that CDC2aAt, CDC2bAt, and CYCB1,1 only operate in mitotically dividing cells, whereas CKS1At may play a role in both the mitotic and endoreduplication cycles.

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