XVI International Botanical Congess

Genetic basis of modern wheat cultivars is quite limited as it has been proven by several studies. Older landraces could serve as a reservoir of desirable genes including those coding for proteins driving dought quality. We have evaluated over 50 genotypes using PCR and locus-specific primers to detect length variability of ?-gliadine psuedogene (1B chromosome), ?,? gliadine (6th homeolog. gr.), x and y loci of gluteine loci. Very high variability was recorded especially in ?-gliadine pseudogene as compared with variability detected in the set of 25 advanced wheat cultivars representing spectrum of standard gliadine loci. We studied as well sequencing variability of 5_ end of ID and 1B loci. Surprisingly variability was found even among the coding sequences of the same allel as they were determined according electrophoretic mobility of the storage proteins in polyacrylamid gel. Using this technique, new alel was discovered in the multi-lines cv. Erythrosperm , which has been studied in more detail.