ibclogo XVI International Botanical Congess

Abstract Number: 4663
Poster No. = 303


COMPARISON OF KARYOTYPE AND RDNA-DISTRIBUTION IN SOMATIC CHROMOSOMES OF BOWENIA SPECIES (STANGERIACEAE, CYCADALES).


Goro Kokubugata*, Katsuhiko Kondo^, Gary W. Wilson#, Lou M. Randall~, *Tsukuba Bot. Gard., Natn. Sci. Mus, ^Lab. Plant Chrom. Gene Stock, Fac.Sci., Hiroshima Univ., #Queensland Herbarium, Queensland Dept. Environment, ~Cycad Connections.


Somatic chromosome at mitotic metaphase of Bowenia serrulata, B. spectabilis and B. sp. 'Tinaroo' is investigated by the standard aceto-orcein staining method and the fluorescent in situ hybridization method (FISH) with ribosomal DNA (rDNA) probe. Bowenia serrulata, B. spectabilis and B. sp. 'Tinaroo' each have a chromosome number of 2n=18. The karyotype of B. serrulata exhibits 10 median-centromeric chromosomes, while B. spectabilis and B. sp. 'Tinaroo' exhibit 8 median-centromeric chromosomes. Using FISH, B. serrulata, B. spectabilis and B. sp. 'Tinaroo' show a hybridization signal on the satellite of the short arm of two submedian-centromeric chromosomes. However, the other hybridization signal pattern is different among B. serrulata, B. spectabilis and B. sp. 'Tinaroo'.

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