ibclogo XVI International Botanical Congess

Abstract Number: 4600
Poster No. = 2421


ARNICA MONTANA AND ARNICA FOLIOSA: IN VITRO CULTURE AND PRODUCTION OF SECONDARY METABOLITES


P.P. Kalynyak1, T. B. Kalynyak1, I. Kostenyk2, Ya. B. Blume2, 1Laboratory of Ecology and Biotechnology, Ternopil Pedagogical University, Ukraine,2 Institute of Cell Biology and Genetic Engineering, Kyiv, Ukraine


Arnica sp. plants are widely used in the pharmaceutical industry and the traditional medicine due to the presence of pharmacologically active compounds: sesquiterpene lactones, flavonoids and essential oils. In a view of there protected status in the Ukraine, increasing industrial demand, in vitro conservation of the local genotypes is very important.On the basis of explants, colour of callus, several strains of A. montana (cutting explants: a light cream and purple strain) and A. foliosa (a white root strain and a deep green strain, leaf explants) were established. Adequate growth of above lines was observed on the Gamborg's basal medium (B5) supplemented with 2-4 mg/L NAA, 0.2-1 mg/L Kin. Analysis of secondary metabolites by TLC and paper chromatography of extracts from A. foliosa strains revealed: chlorogenic acid, esters of caffeic and chuinic acids, allantolactones and isoallantolactones.The optimum conditions for protoplasts isolation from A. foliosa have been determined.

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